Detection of "non-precipitating" Antibodies in Sera of Individuals Allergic to Ragweed Pollen by an in Vitro Method

نویسندگان

  • J. Gordon
  • B. Rose
  • A. H. Sehon
چکیده

A hemagglutination test capable of demonstrating antibodies in sera of ragweed-sensitive individuals has been described in detail. This test involves coupling of the antigen to rabbit erythrocytes via stable azo bonds. The antigen-coated cells are then suspended in the serum which is suspected to contain the homologous antibodies. The test has been shown to be specific and highly sensitive. Precipitating antibodies to ragweed pollen extract produced in experimental animals can be demonstrated in dilutions as high as 10(5) to 10(7), while antibodies to ragweed in allergic sera are detected only in dilutions of the order of 10(3). Positive results were obtained with all sera from treated or non-treated allergic individuals containing skin-sensitizing and/or blocking antibodies. Absorption of these sera with antigen-coated erythrocytes yielded a supernatant devoid of either skin-sensitizing, blocking, or hemagglutinating capacity. It is concluded that the hemagglutinating factor has the properties of a divalent antibody.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Prevalence of Saffron Pollen Allergy in Saffron Workers of Khorasan (Iran) in 2002

Iran is the main producer of Saffron in the world and a great number of Khorasan (the largest province of Iran) are involved in growing this plant. Recently, saffron pollen has found great importance due to inducing allergic reactions. In this study, the prevalence and clinical symptoms of saffron pollen allergy and specific IgE level in saffron workers have been studied. For this purpose, 167 ...

متن کامل

Ragweed-allergic subjects have decreased serum levels of chemokines CCL2, CCL3, CCL4 and CCL5 out of the pollen season.

CC-chemokines are important mediators of the allergic responses and regulate the cell trafficking. The aim of this study was to examine the serum levels of CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β and CCL5/RANTES, and to determine whether there are differences between ragweed-allergic subjects and healthy individuals out of the pollen season. Peripheral blood samples were collected from 24 subjects...

متن کامل

Serum Antisperm Antibodies in Fertile and Infertile Individuals

: Presence of antisperm antibodies (ASAs) in infertility and their adverse effects on fertility is a matter of controversy. The aims of this study were to determine the percentage of antibody positive sperms and rate of ASA positive sera in both fertile and infertile men and women, and to find the association between these antibodies and infertility. Methods: This study consisted of 29 fertile ...

متن کامل

Ambrosia artemisiifolia (ragweed) in Germany – current presence, allergological relevance and containment procedures

Ambrosia artemisiifolia (ragweed) is a neophyte in Europe and Germany, which originated from the United States of America. In the USA the rate of sensitization against ragweed equals that of grass pollen, and without containment the rate of allergic sensitizations against ragweed pollen will clearly increase. Currently, the most frequent sensitizations in Germany are against grass pollen, follo...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 108  شماره 

صفحات  -

تاریخ انتشار 1958